Staphylococcus aureus is one of the most important bacteria in Hospital and community-acquired infections. Antibiotic resistance that is caused by this bacterium to be a major problem in health care. The purpose of this study was to determine vancomycin minimum inhibitory concentration by E-test in Staphylococcus aureus isolated in two tertiary Hospitals in the city of Sanandaj. This study cross-sectional study was performed on 88 samples Staphylococcus aureus isolated from clinical specimens of patients admitted to Toohid and Besat Hospitals in Sanandaj city (April to March 2011). Strains identification was done by Microbiology standard methods. Antibiotic susceptibility testing by disk diffusion and vancomycin minimum inhibitory concentration by E-test was performed. In this study 40 S. aureus strains from Toohid and 48 strains from Besat Hospitals was collected. Between 88 isolates of S. aureus, minimum inhibitory concentration ranges was varied of 0.19-1.5 milligrams per liter and according to standards were all strains were sensitive to vancomycin (minimum inhibitory concentration ≤ 2). In this study resistance strains to vancomycin not found. The results of this research can play an important role in resistance to vancomycin assess in the health care system in the Kurdistan province, as well as for the correct application of the antibiotic treatment of infections.