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:: year 7, Issue 30 (3-2016) ::
3 2016, 7(30): 40-44 Back to browse issues page
Rapid detection of Lipocalin-2 in prostate cancer patient's serum by gold nanoparticles
Reza Nekouian 1, Bahareh Sadat Rasouli2
2- Medical Biotechnology Department, School of Allied Medicine, Iran University of Medical sciences, Tehran, Iran
Abstract:   (6358 Views)

Background and Aim: Broad applications of nanomaterials in the field of biology and medicine helped scientists to find a way to change many clinical and traditional diagnostic and prognostic methods. Cancer nanotechnology is a new area of medical nanobiotechnology research which has a great impact on detection and diagnosis of cancer. Prostate cancer is the third common cancer in Iran and Lipocalin-2 has an important role in cancer progression.

Materials and Methods: Gold nanoparticles (AuNPs) with a diameter of ~35nm were prepared by citrate reduction of Gold (III) chloride hydrate (HAuCl4) based on the method introduced by Turkevich et al. Anti-Lipocalin2 were conjugated to gold nanoparticles to design a colorimetric biosensor for detection of Lipocalin2.

Results: The color of the gold nanoparticle were changed from blue to red. This color shift is indicator of nanoparticles aggregation due to binding of Lipocalin2.

Conclusion: This colorimetric biosensor could be used as an accessible, rapid Lipocalin2 detection method in prostate cancer diagnosis.

Keywords: Prostate cancer, Lipocalin2, Gold nanoparticles, Antibody
Full-Text [PDF 447 kb]   (2740 Downloads)    
Type of Study: Applicable | Subject: آسیب شناسی بالینی و تشریحی
Received: 2016/03/26 | Accepted: 2016/03/26 | Published: 2016/03/26
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Nekouian R, sadat Rasouli B. Rapid detection of Lipocalin-2 in prostate cancer patient's serum by gold nanoparticles. 3 2016; 7 (30) :40-44
URL: http://labdiagnosis.ir/article-1-147-en.html

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year 7, Issue 30 (3-2016) Back to browse issues page
Laboratory and Diagnosis
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