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Hemoglobin A1c and g-Hb measurement and correction in the diagnosis and monitoring of diabetes
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Nader Vazifeh Shiran   1, Maryam sadat Shaker Taheri1 |
| 1- Shahid Beheshti Medical University, Tehran, Iran |
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Abstract: (53 Views) |
Hemoglobin A1c is the percentage of total hemoglobin that is bound to the NH3+ amino acid valine of the initial beta-globin chain during erythropoiesis and the 120-day lifespan of RBCs, and by removing the positive charge, it induces a negative charge and, as a result, its rapid movement in hemoglobin electrophoresis. The normal value of Hb-A1c is about 6%, and its increase is an indicator of diabetes, treatment monitoring, and the approximate blood sugar level of the patient over the past 3 months. Since in hemoglobinopathies, one or both beta chains may mutate to another globin, as a result, the amount of hemoglobin A decreases and is added to the variant hemoglobin, and thus the amount of Hb-A1c also decreases. In homozygous cases such as S/S, the amount of hemoglobin A and consequently Hb-A1c reaches zero and Hb-S1c is formed instead, which must be corrected when using HPLC and capillary electrophoresis. In this article, methods for correcting Hb-A1c with the above two methods, interfering factors, and alternative methods will be discussed.
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| Keywords: Diabetes, Hemoglobin A1c, AGE, Capillary Electrophoresis, HPLC, Fructosamine, Glycated Albumin |
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Full-Text [PDF 833 kb]
(43 Downloads)
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Type of Study: Review |
Subject:
خون شناسی (هماتولوژی)
Received: 2024/12/28 | Accepted: 2024/12/29 | Published: 2024/12/29
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