Background and Aim: Broad applications of nanomaterials in the field of biology and medicine helped scientists to find a way to change many clinical and traditional diagnostic and prognostic methods. Cancer nanotechnology is a new area of medical nanobiotechnology research which has a great impact on detection and diagnosis of cancer. Prostate cancer is the third common cancer in Iran and Lipocalin-2 has an important role in cancer progression.

Materials and Methods: Gold nanoparticles (AuNPs) with a diameter of ~35nm were prepared by citrate reduction of Gold (III) chloride hydrate (HAuCl₄) based on the method introduced by Turkevich et al. Anti-Lipocalin2 were conjugated to gold nanoparticles to design a colorimetric biosensor for detection of Lipocalin2.

Results: The color of the gold nanoparticle were changed from blue to red. This color shift is indicator of nanoparticles aggregation due to binding of Lipocalin2.

Conclusion: This colorimetric biosensor could be used as an accessible, rapid Lipocalin2 detection method in prostate cancer diagnosis.